|
|
|
荷斯坦乳牛乳腺和乳腺上皮细胞中Toll样受体及
辅助因子编码基因的检测
|
|
|
|
王亨1,2，孟霞3，邱昌伟1，吴培福1，韩超1，齐长明1
|
|
|
|
(1.中国农业大学 动物医学院 临床兽医系，北京100094；2.扬州大学 兽医学院，江苏 扬州225009；
3.中国农业大学 生物学院 微生物学与免疫学系，北京100094)
|
|
|
|
摘要：
|
|
参照牛TLR4、TLR2、CD14、MD-2基因序列设计了相应基因的引物。采用RT-PCR技术检测了体外培养的荷斯坦乳牛乳腺和乳腺上皮细胞中Toll样受体TLR4、TLR2及辅助因子CD14、MD-2基因。结果显示，乳腺上皮细胞中存在TLR4、TLR2、CD14和MD-2四个基因的表达，而乳腺中除MD-2未检测到外，其余3个基因均扩增成功。说明该受体及辅助因子可能参与了乳腺的先天性免疫防御。该研究为探讨乳腺的先天性免疫及乳腺上皮细胞在乳腺先天性免疫中的作用奠定了基础。 |
|
关键词：
|
|
牛；乳腺；Toll样受体；辅助因子；先天性免疫 |
|
中图分类号：S 852.4：Q 503 文献标识码：
A 文章编号：1673-4696(2007)10-0882-05 |
|
|
Detection of genes encoding Toll-like receptors and accessory molecules
in mammary gland and mammary epithelial cells from Holstein cows |
|
|
|
WANG Heng1,2,MENG Xia3,QIU
Chang-wei1,WU Pei-fu1,HAN Chao1,QI
Chang-ming1 |
|
|
|
(1.Department of Clinical Veterinary Science,College of Veterinary Medicine,China Agricultural University,Beijing
100094,China;2.College of Veterinary Medicine,Yangzhou University,Yangzhou 225009,China;3.Department of
Microbiology and Immunology,College of Biological Sciences,China Agricultural University,Beijing 100094,China) |
|
|
Abstract:Primers to amply TLR4,TLR2,CD14 and MD-2 genes were designed according to the rela-ted bovine sequences and were applied to detect the genes in mammary gland and mammary epithelial cells from Holstein cows cultured in vitro by RT-PCR.The four genes were expressed in the bovine mammary epithelial cells.With the exception of MD-2 gene,the remaining genes were detected in bovine mammary tissues.Innate immunity defense may be reinforced with the presence of the receptors and accessory mole-cules.These results provided a foundation for understanding the innate immunity of mammary gland and for further studies on the role of mammary epithelial cells in innate immunity of mammary gland. |
|
|
|
Key words: cattle;mammary gland;Toll-like receptor;accessory molecule;innate immunity |
|
|
|
|
|
《中国兽医科学》 ©
版权所有
Chinese Veterinary Science
zgsykx@zgsykx.com |
