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绵羊血浆中氟甲喹及其代谢产物7-羟基氟甲喹
浓度的RP-HPLC测定
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周庆
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（浙江大学 实验动物中心，浙江 杭州310058）
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摘要：
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利用C8色谱柱(4.6mm×150mm,5μm)，以V(乙腈)∶V(15mmol/L KH2PO4)缓冲液=39∶61为流动相，在320nm紫外光下，利用反相高效液相色谱法（RP-HPLC）测定了绵羊血浆中氟甲喹及其代谢产物7-羟基氟甲喹的浓度。结果显示，氟甲喹和7-羟基氟甲喹分别在20～10000ng/mL和20～500ng/mL范围内线性关系良好（r=0.999和r=0.998），平均绝对回收率分别为84.3%±6.7%和67.5%±56%，日内与日间精密度均小于8.3%，最低检测限均为10ng/mL；内源性物质不干扰测定。表明，RP-HPLC的工作曲线稳定，灵敏度高，操作步骤简单，可用于绵羊血浆中氟甲喹及其代谢产物7-羟基氟甲喹浓度的测定和药动学考察。 |
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关键词：
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氟甲喹；7-羟基氟甲喹；反相高效液相色谱 |
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中图分类号：R 963 文献标识码：
A 文章编号：1673-4696(2007)10-0905-04 |
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Determination of flumequine and its metabolite 7-hydroxyflumequine
in sheep plasma by RP-HPLC |
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ZHOU Qing |
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（Centre for Laboratory Animal,Zhejiang University,Hangzhou 310058,China） |
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Abstract: The concentrations of flumequine and its metabolite 7-hydroxyflumequine in sheep plasma were determined by the reversed phase high performance liquid chromatography(RP-HPLC).For the RP-HPLC analysis,a reversed phase C8 column was used for the separation of flumequine and 7-hydroxyflumequine,mobile phase was acetonitrile-15mmol/L KH2PO4(39∶61,V/V),and the detector was operated at the 320nm wavelength.The chromatography was not interfered by the components of the plasma.The calibration curve for flumequine and 7-hydroxyflumequine assay exhibited excellent linear relationship with a correlation coefficients 0.999 and 0.998 in the concentration ranges of 20-10000ng/mL and 20-500ng/mL,respectively.The absolute recovery of flumequine and 7-hydroxyflumequine was 84.3%±6.7% and 67.5%±5.6%,respectively.The intra-day and inter-day precisions were less than 8.3%.The limit of detection was 10ng/mL.These results showed that the method was simple and accurate,and could be used to determine flumequine and 7-hydroxyflumequine from sheep plasma samples. |
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Key words: flumequine;7-hydroxyflumequine;RP-HPLC |
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