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蕨麻多糖对小鼠免疫细胞信号转导相关分子的影响
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胡庭俊1 ，陈炅然2，张霞3，郑立颖3，程富胜2,郑荣梁4
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（1.广西大学 动物科技学院,广西 南宁530004;2.中国农业科学院 兰州畜牧与兽药研究所，
甘肃 兰州730050；3.甘肃农业大学 动物医学院，甘肃 兰州730070；
4.上海高校一氧化氮及炎症医学E-研究院,上海201203）
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摘要：
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以50、100、200、400μg/mL的蕨麻多糖对小鼠脾淋巴细胞在体外分别孵育4、8、12、24h，测定了细胞培养液中一氧化氮（NO）、6-酮-PGF1α及血栓素B2(TXB2)的水平和细胞内cGMP的含量，测定了加入蕨麻多糖后2、5、10min时细胞内游离钙离子(Ca2+)的浓度。结果显示，蕨麻多糖能明显促进小鼠脾淋巴细胞分泌一氧化氮，显著升高小鼠脾淋巴细胞内cGMP及钙离子的水平，对细胞内TXB2的产生有抑制作用，能够调整花生四烯酸体系向6-酮-PGF1α一端偏移。提示蕨麻多糖能够增强脾淋巴细胞的免疫作用。 |
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关键词：
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蕨麻多糖；免疫细胞；信号转导 |
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中图分类号：S 853.74文献标识码：
A 文章编号：1673-4696(2007)10-0909-05 |
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Effect of Potentilla anserine polysaccharide on signal transduction-
related molecules in murine immunocytes |
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HU Ting-jun1,CHEN Jiong-ran2,ZHANG
Xia3,ZHENG Li-ying3,
CHENG Fu-sheng2,ZHENG Rong-liang4 |
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(1.College of Animal Science and Technology,Guangxi University,Nanning 530004,China;2.Lanzhou Institute of
Animal Sciences & Veterinary Pharmaceutics,Chinese Academy of Agricultural Sciences,Lanzhou 730050,China;
3.College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,China;4.Division of Nitric Oxide
and Inflammatory Medicine,E-Institutes of Shanghai Universities,Shanghai 201203,China) |
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Abstract: Murine splenic lymphocytes were incubated in medium alone or medium containing Potentilla anserine polysaccharide(PAP) in the concentrations of 50μg/mL,100μg/mL,200μg/mL or 400μg/mL for 4h,8h,12h or 24h in vitro,respectively.The supernatants at different culture periods were analyzed for changes in levels of nitric oxide(NO),6-keto-prostaglandin F1α and thromboxane B2(TXB2).The cells were collected to determine contents of cGMP.The changes in levels of the concentrations of cytoplasmic free Ca2+ were measured at minute 2，5 and 10 post-incubation,respectively.PAP increased the production of NO and the concentration of intracellular free calcium in splenic lymphocytes and raised the levels of cGMP and 6-keto-PGF1α of murine splenic lymphocytes in vitro.The levels of TXB2 dropped in the cells treated with PAP.PAP was able to regulate arachidonic acid signal transduction of immunocytes.These results showed that PAP was able to enhance immune functions of the splenic lymphocytes. |
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Key words: Potentilla anserine polysaccharide;immunocytes;signal transduction |
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