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阿维菌素对鸽脑组织GABAA受体结合作用的影响
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程广东1,2，徐世文1，李术1
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(1.东北农业大学 动物医学学院，黑龙江 哈尔滨150030；
2.佳木斯大学 生命科学学院，黑龙江 佳木斯154007)
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摘要：
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在试验组日粮中按40mg/kg添加阿维菌素(AVM)，对照组饲喂不添加AVM的常规日粮，利用3H-蝇蕈醇（3H-muscimol）与鸽脑组织γ-氨基丁酸（GABA）均能与GABAA受体结合的特性，应用放射免疫法测得3H-蝇蕈醇与王鸽大脑GABAA受体结合的平衡解离常数(kd)和最大结合量(Bmax)分别为（44.62±207）nmol/L和（3.31±0.10）pmol/mg。结果表明，AVM可使3H-muscimol与王鸽大脑组织GABAA受体结合量增大，AVM可促进GABA的释放，增强GABA的抑制作用。 |
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关键词：
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阿维菌素；美国王鸽；脑组织；γ-氨基丁酸A受体 |
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中图分类号：R 994.6 文献标识码：
A 文章编号：1673-4696(2007)11-0974-04 |
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Effect of avermectin on GABAA receptor in cerebrum of King pigeons |
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CHENG Guang-dong1,2，XU Shi-wen1，LI
Shu1 |
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（1.College of Veterinary Medicine,Northeast Agricultural University,Harbin 150030，China；
2.College of Life Science,Jiamusi University,Jiamusi 154007，China） |
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Abstract:King pigeons were treated with a diet containing 0 or 40mg/kg of avermectin. Dissociation constant (kd) and maximal binding capacity (Bmax) of 3H-muscimol to GABAA receptor were determined in vitro using a radio-immune assay. Results showed that the kd and Bmax was (44.62±2.07）nmol/L and （331±0.10）pmol/mg respectively in the reference group. Avermectin increased Bmax of 3H-muscimol to GABAAreceptors. The increase in Bmax could result in enhanced release of GABA and the central inhibitory effect of the transmitter. |
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Key words: avermectin;King pigeon;cerebral tissue;GABAA receptor |
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