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FSH对仔猪睾丸支持细胞增殖和GDNF表达的调节
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孙燕1，2，王鲜忠1，2，吴建云2，白汝岚2，张家骅1，2
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（1.西南大学 牧草与草食家畜重点实验室，重庆400716；2.西南大学 动物科技学院，重庆400716）
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摘要：
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以原代培养的仔猪睾丸支持细胞（SC）为试验模型，研究了FSH对SC胶质细胞源性神经营养因子(GDNF)表达及其增殖的影响。结果显示，外源性添加FSH可促进GDNF蛋白表达，而且这种促进作用具有浓度和时间依赖关系，GDNF蛋白水平随着FSH浓度的增加而升高，当FSH浓度为50ng/mL时，GDNF水平达到最高，然后逐渐降低；FSH（50ng/mL）作用30min，可显著促进GDNF蛋白的表达(P<005)，当FSH作用1h时，GDNF蛋白水平达到最高，随后逐渐降低。FSH可以时间-剂量依赖性促进SC增殖细胞核抗原（PCNA）的表达，FSH（50ng/mL）作用30min时，PCNA的表达显著增加(P<0.05)，作用1h达极显著水平(P<0.01)，至2h时，PCNA蛋白的表达水平达到最高，随后逐渐降低。提示，FSH可在体外条件下通过刺激SC表达GDNF进而促进其增殖。 |
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关键词：
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促卵泡素；睾丸支持细胞；胶质细胞源性神经营养因子（GDNF）；增殖细胞核抗原 |
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中图分类号：S 814.1 文献标识码：
A 文章编号：1673-4696(2007)11-0982-05 |
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Regulation of FSH on proliferation of piglet testis sertoli cell
and expression of GDNF |
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SUN Yan1,2,WANG Xian-zhong1,2,WU
Jian-yun2,BAI Ru-lan2,ZHANG Jia-hua1,2 |
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(1．Chongqing Key Laboratory of Forage & Herbivore,Southwest University,Chongqing 400716,China；
2．College of Animal Science and Technology,Southwest University,Chongqing 400716，China) |
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Abstract: Primary piglet sertoli cells(SCs) were used to analyze the effects of FSH on the proliferative potential and GDNF expression of SCs.The FSH-stimulated GDNF expression in the cultured SCs was time- and concentration-dependent,that was the expression of GDNF protein reached its highest level when the concentration of FSH was 50ng/mL.The protein expression was increased significantly when the sti-mulating time was 30min and reached a peak level at hour 1,then declined gradually.FSH increased expression of proliferating cell nuclear antigen(PCNA) in SCs.Expression level of PCNA protein increased significantly at 30th min and reached a peak at hour 2,then declined gradually.It was concluded that FSH was able to enhance proliferation of SCs in time- and dose-dependent manners by stimulating expression of GDNF. |
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Key words:FSH;SC;glial cell line-derived neurotrophic factor;proliferating cell nuclear antigen |
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