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嗜水气单胞菌Lamp检测方法的建立及应用
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程天印，刘洵，常小斌
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(湖南农业大学 动物医学院，湖南 长沙410128)
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摘要：
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基于嗜水气单胞菌气溶素(aerolysin) 基因的序列,设计了1套引物，通过条件优化，成功建立了针对致病性嗜水气单胞菌的环媒恒温基因扩增（Lamp）检测法。采用Lamp法对病原菌进行了扩增，并对病鱼血样进行了检测。结果表明，该法只检出致病性嗜水气单胞菌。对不同浓度的细菌悬液扩增结果表明，Lamp检测嗜水气单胞菌的最低菌液浓度为1.4～14/μL。 |
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关键词：
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嗜水气单胞菌;气溶素基因;环媒恒温基因扩增检测法 |
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中图分类号：S 917.1 文献标识码：
A 文章编号：1673-4696(2007)12-1013-04 |
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Establishment and application of loop-mediated isothermal
amplification for detection of Aeromonas hydrophila |
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CHENG Tian-yin，LIU Xun，CHANG Xiao-bin |
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(College of Veterinary Medicine,Hunan Agricultural University,Changsha 410128,China)
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Abstract: A set of oligonucleotide primers were designed and synthesized based on the published aerolysin gene sequence of Aeromonas hydrophila，and the loop-mediated isothermal amplification(Lamp) for detection of A.hydrophila was developed after optimizing the factors.The pathogenic bacteria and clinical samples were detected with the Lamp，and the sequence from the Lamp was analyzed.The results showed that the method was valid for detection of pathogenic strains of A.hydrophila.The Lamp detection limit for A.hydrophila was 1.4-14 A.hydrophila per μL. |
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Key words: Aeromonas hydrophila;aerolysin gene;Lamp method |
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