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3种基因型鸡传染性支气管炎病毒免疫对
tl/CH/LDT3/03株的保护性
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李成仁1,2，张晓楠1,2，王钰1,3，韩宗玺1，刘乔然1，邵昱昊1，刘胜旺1，孔宪刚1
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（1.中国农业科学院 哈尔滨兽医研究所 兽医生物技术国家重点实验室 禽传染病研究室，黑龙江
哈尔滨 150001；2.东北农业大学，黑龙江 哈尔滨 150030；3.黑龙江八一农垦大学，黑龙江 大庆163319）
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摘要：
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以鸡传染性支气管炎病毒(IBV) S1基因比较为基础，分析了IBV tl/CH/LDT3/03型毒株与中国其他流行IBV毒株之间的关系，选择5株代表3种基因型的毒株进行动物免疫攻毒试验，以发病率、死亡率、抗体产生及在不同脏器中的病毒检出率作为指标来评价异种毒株对tl/CH/LDT3/03株的保护性。结果显示，同种致弱毒株对tl/CH/LDT3/03强毒株具有良好的保护性，而异种毒株对tl/CH/LDT3/03强毒株的保护性低。 |
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关键词：
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鸡传染性支气管炎病毒；S1基因；交叉保护 |
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中图分类号：S 852.659.6 文献标识码：
A 文章编号：1673-4696(2007)12-1024-05 |
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Protection of chickens immunized with three genotypes of avian infectious
bronchitis viruses against IBV strain tl/CH/LDT3/03 |
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LI Cheng-ren1,2,ZHANG Xiao-nan1,2,WANG
Yu1,3,HAN Zong-xi1,LIU Qiao-ran1,
SHAO Yu-hao1,LIU Sheng-wang1,KONG Xian-gang1 |
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(1.National Key Laboratory of Veterinary Biotechnology/Division of Avian Infectious Diseases,Harbin Veterinary
Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150001,China;2.Northeast Agricultural
University,Harbin 150030,China;3.Heilongjiang August First Land Reclamation University,Daqing 163319,China) |
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Abstract: By comparison of S1 gene nucleotide sequences among avian infectious bronchitis viruses(IBV),genotype relationship between IBV tl/CH/LDT3/03 strain and the other IBV isolates in China was analyzed.With morbidity, mortality, antibody level and virus re-isolation in different tissues of SPF chickens as indexes,the protection of chickens immunized with 5 IBV strains representing 3 genotypes against high virulent IBV tl/CH/LDT3/03 strain was evaluated.The result showed that the attenuated homologous strains were superior to the heterogeneous strains in protection of chickens against the IBV strain tl/CH/LDT3/03. |
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Key words: avian infectious bronchitis virus;S1 gene;cross protection |
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