| |
|
 |
|
|
|
应用BALB/c小鼠评价口蹄疫灭活疫苗的免疫效力
|
|
|
|
智晓莹1,2，张永光2，吴润1，王永录2，潘丽2，方玉珍2，蒋守田2，
张维德2，黄振华2，周鹏2，王占伟2，吕建亮2,刘力宽2
|
|
|
|
（1.甘肃农业大学 动物医学院，甘肃 兰州730070；2.中国农业科学院 兰州兽医研究所 家畜疫病
病原生物学国家重点实验室 农业部畜禽病毒学重点实验室 国家口蹄疫参考实验室，甘肃 兰州730046）
|
|
|
|
摘要：
|
|
用不同剂量的口蹄疫灭活疫苗免疫3组雌性BALB/c小鼠，同时设空白对照组，每组8只。免疫后每7d采血一次，用液相阻断ELISA（LPB-ELISA）检测血清抗体水平；第28d用800LD50同源强毒攻击，攻毒后36h，每组随机选取3只BALB/c小鼠，采全血，分别用每只BALB/c小鼠全血注射12只乳鼠，每组共注射36只乳鼠，以乳鼠试验判定BALB/c小鼠的病毒血症和攻毒保护情况。结果表明，免疫组BALB/c小鼠均可产生特异性抗体，保护率分别为75.0%、63.9%、36.1%；对照组小鼠血清抗体为阴性。提示，BALB/c小鼠可以用来评价口蹄疫灭活疫苗的免疫效力。 |
|
关键词：
|
|
口蹄疫灭活疫苗；BALB/c小鼠；免疫效力；病毒血症 |
|
中图分类号：S 852.659.6
文献标识码：
A 文章编号：1673-4696(2007)12-1050-03 |
|
|
Evaluation of efficacy of inactivated vaccine against
foot-and-mouth disease in BALB/c mice |
|
|
|
ZHI Xiao-ying1,2,ZHANG Yong-guang2,WU
Run1,WANG Yong-lu2,PAN Li2,FANG Yu-zhen2,
JIANG Shou-tian2,ZHANG Wei-de2,HUANG Zhen-hua2，ZHOU
Peng2,WANG Zhan-wei2,
L Jian-liang2,LIU Li-kuan2 |
|
|
|
(1.College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,China;2.National Foot-and-Mouth
Disease Reference Laboratory/ Key Laboratory of Animal Virology of the Ministry of Agriculture/State Key
Laboratory of Veterinary Etiological Biology/Lanzhou Veterinary Research Institute,Chinese Academy of
Agricultural Sciences,Lanzhou 730046,China) |
|
|
Abstract: Thirty-two adult female BALB/c mice,8 per group,were immunized with different doses of the inactivated foot-and-mouth disease(FMD) type O vaccine and the control group was set.Whole blood of all the mice were collected on day 7,14,21 and 28 post-immunization and FMDV-specific neutralizing antibody(NAb) levels were examined by liquid-phase blocking sandwich ELISA(LPB-ELISA).On day 28,all the mice were challenged with 800 LD50 of the prevalent FMDV from the same origin.Whole blood from 3 mice in each group were collected at hour 36 post-infection and injected into 36 sucking mice totally to assess protection in the BALB/c mice.In results,FMDV-specific NAb was found in the immunized mice.The protection rates for the mice in the three experimental groups were 75.0%,63.9% and 36.1%,respectively.The mice in the control group had no FMDV-specific NAb response to the vaccine.The results revealed that BALB/c mice could be used to evaluate efficacy of inactivated vaccines against FMD. |
|
|
|
Key words: inactivated vaccine against FMD;BALB/c mice;immune efficacy;viremia |
|
|
|
|
|
《中国兽医科学》 ©
版权所有
Chinese Veterinary Science
zgsykx@zgsykx.com |
|
