| |
|
 |
|
|
|
羊源肠球菌溶血素特性分析及其对兔
红细胞作用的观察
|
|
|
|
康立超1,2，马勋1，王静梅1，薄新文2
|
|
|
|
(1.石河子大学 动物科技学院，新疆 石河子832003；
2.新疆农垦科学院 新疆兵团绵羊繁育生物技术重点实验室，新疆 石河子832000)
|
|
|
|
摘要：
|
|
对致羔羊脑炎肠球菌E129-3菌株溶血素进行检测分析，并利用光学显微镜和电子显微镜观察其对兔红细胞的作用，探讨了肠球菌溶血素的特性及溶血类型。结果表明，小白鼠、兔、犬红细胞对该溶血素最敏感，牛红细胞不敏感。该溶血素可以被DTT、Ca2+活化，被蛋白酶K、EDTA、Zn2+、Cu2+抑制。在培养后2h，细胞浆内着色不一致或密度降低，5h时红细胞数量明显减少，细胞膜较完整。提示，肠球菌溶血素为成孔蛋白类溶血素。 |
|
关键词：
|
|
肠球菌；溶血素；特性；红细胞 |
|
中图分类号：S 852.611 文献标识码：
A 文章编号：1673-4696(2007)12-1069-05 |
|
|
Characteristic analysis of hemolytic toxin of Enterococcus from
sheep and its effect on rabbit erythrocytes |
|
|
|
KANG Li-chao1,2,MA Xun1,WANG
Jing-mei1,BO Xin-wen2 |
|
|
|
(1.College of Animal Science and Technology,Shihezi University,Shihezi 832003,China;2.Xinjiang Army Group
Key Laboratory of Sheep Reproduction Biotechnology,Xinjiang Academy of Agriculture and Reclamation,
Shihezi 832000,China) |
|
|
Abstract:Hemolysin from Enterococci (E129-3) hemolytic strains causing encephalitis in lamb was detected and analyzed,effect of the hemolysin on rabbit erythrocytes was observed by the optical microscopy and electron microscopy and the characteristics of Enterococci hemolysis and hemolytic types were probed.In result,erythrocytes from mouse,rabbit,and dog were sensitive to the hemolysin,while those from cattle were less susceptible to the hemolysin.This hemolysin could be activated by 1,4-Dithiothreitol (DTT),Ca2+ ,but it could be restrained by proteinase K,EDTA,Zn2+ and Cu2+.Inconsistent coloring occured or density obviously decreased in the cytoplasm at hour 2 post-culturing.The blood cell count reduced significantly at hour 5 post-culturing.The result shows that hemolytic toxin of Enterococcus is hole protein class hemolysin. |
|
|
|
Key words: Enterococcus;hemolysin;characteristic;erythrocyte |
|
|
|
|
|
《中国兽医科学》 ©
版权所有
Chinese Veterinary Science
zgsykx@zgsykx.com |
|
