|
|
|
复方“连黄”对金黄色葡萄球菌耐药基因femA的影响
|
|
|
|
王晓波1，鞠玉琳1，李希洙2，朴信映2
|
|
|
|
（1.延边大学 农学院,吉林 龙井133400;2.韩国兽医科学检疫院,韩国 首尔135230）
|
|
|
|
摘要：
|
|
采用聚合酶链式反应（PCR）扩增金黄色葡萄球菌耐药基因femA；通过耐药基因失活试验，研究了中药复方“连黄”对金黄色葡萄球菌耐药基因femA的影响，探讨了中药“连黄”降低金黄色葡萄球菌对β-内酰胺类药物耐药性的作用机理。结果显示，中药“连黄”作用前后金黄色葡萄球菌的耐药基因femA在23～72碱基区发生较大改变，对应的氨基酸序列也发生改变，从而导致femA基因失活。 |
|
关键词：
|
|
复方“连黄”；聚合酶链式反应；femA基因；耐药性 |
|
中图分类号：S 853.74 文献标识码：
A 文章编号：1673-4696(2007)12-1082-04 |
|
|
Effects of Chinese traditional medicine Lianhuang on the drug
resistance gene femA of Staphylococcus aureus |
|
|
|
WANG Xiao-bo1,JU Yu-lin1,LI Xi-zhu2,PIAO
Xin-ying2 |
|
|
|
(1.College of Agriculture,Yanbian University,Longjing 133400,China;2.National Veterinary
Research and Quarantine Service of the Republic of Korea,Seoul 135230,The Republic of Korea) |
|
|
Abstract: To elucidate the mechanism of Chinese traditional medicine Lianhuang degrading the resis-tance of Staphylococcus aureus to β-lactam-like drugs,a drug resistance gene femA of S.aureus was amplified by PCR and the effect of Lianhuang on the gene femA was examined by using the drug resistance gene inactivation test.In result,Lianhuang caused mutation of 23-72bp of the region of the gene femA,which resulted in changes of the corresponding amino acid sequences too.The result showed that the Chinese medicine Lianhuang can inactivate the gene femA. |
|
|
|
Key words: Chinese traditional medicine Lianhuang;polymerase chain reaction(PCR);femA gene;drug resistance |
|
|
|
|
|
《中国兽医科学》 ©
版权所有
Chinese Veterinary Science
zgsykx@zgsykx.com |
