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氯霉素残留伏安免疫测试法的电化学条件
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宋巍巍1,丁明星1,刘国艳2,张挪威1,柴春彦2
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（1.华中农业大学 动物医学院，湖北 武汉430070；2.上海交通大学 农业与生物学院，上海201101）
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摘要：
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在间接竞争ELISA的基础上，建立了以碱性磷酸酶（ALP）-对硝基苯磷酸（pNPP）为反应体系的检测氯霉素(CAP)残留的伏安免疫方法,并对此法的电化学条件进行了详细的探讨。结果，在0.2mol/L的B-R电解质溶液(pH3.0)中，当扫描速度为50mV/s时，伏安法对硝基苯酚（PNP）的灵敏度最佳；且在1.2V处，该物质具有良好的氧化峰形。用此法测试CAP的检测下限达到0.064ng/mL，检测线性范围为0.15～600ng/mL。 |
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关键词：
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氯霉素；伏安免疫法；氧化峰电流；电化学条件 |
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中图分类号：S 859.84 文献标识码：
A 文章编号：1673-4696(2007)12-1090-04 |
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Electrochemical conditions for immunovoltammetry-
detection of chloramphenicol residues |
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SONG Wei-wei1,DING Ming-xing1,LIU
Guo-yan2,ZHANG Nuo-wei1,CHAI Chun-yan2 |
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(1.College of Veterinary Medicine,Huazhong Agricultural University,Wuhan 430070,China；
2.College of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 201101,China) |
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Abstract: To explore immunosensor technique with high sensitivity and specificity for determination of chloramphenicol residues in milk,an immunovoltammetric method was developed by using ALP-pNPP as reacting system based on ciELISA,and conditions for immunovoltammetric determination of chloramphenicol were discussed in detail.The results showed that high sensitivity of the voltammetric determination of PNP was manifested in the supporting solution 0.2mol/L Britton-Robinson buffer(pH3.0) with a scanning rate at 50mV/s and finely-sharped oxidative peak current was shown when the potential was at 1.2V.The chloramphenicol detection limit of the method was 0.064ng/mL within the linear range of 0.15-600ng/mL. |
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Key words: chloramphenicol;immunovoltammetry;oxidative peak current;electrochemical condition |
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