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朊毒体蛋白的构象及其特性的研究进展
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陈豪泰1，刘永生1，张杰1，吴润2，刘湘涛1
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(1.中国农业科学院 兰州兽医研究所 家畜疫病病原生物学国家重点实验室 农业部畜禽病毒学重点
实验室，甘肃 兰州730046；2.甘肃农业大学 动物医学院，甘肃 兰州730070)
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摘要：
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概述了朊毒体(包括构象不同的细胞型朊毒体和痒病型朊毒体)蛋白的构象特征、朊毒体及其突变体的特性，阐明了PrPsc 是由PrPc通过构象转变而成的，不同物种PrP的三维结构有相似的球形结构域（125～228位）和N-末端无规卷曲尾，其球形结构域均由3个螺旋（144～154、173～194和200～228位）以及1对反平行的β-折叠（128～131和161～164）组成，但其结构和表面电荷分布存在一些区别。 |
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关键词：
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朊毒体蛋白；构象；特性 |
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中图分类号：S 852.659.7 文献标识码：
A 文章编号：1673-4696(2007)12-1094-05 |
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Progress in conformation and property of prion proteins |
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CHEN Hao-tai1，LIU Yong-sheng1， ZHANG
Jie1，WU Run2， LIU Xiang-tao1 |
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(1.State Key Laboratory of Veterinary Etiological Biology/Key Laboratory of Animal Virology of the
Ministry of Agriculture/Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,
Lanzhou 730046,China;2.College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,China) |
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Abstract: Prion proteins including the cellular isoform of prion protein(PrPc) and the scrapie isoform of prion protein(PrPsc) were reviewed in the aspects of conformational characteristics,and properties of prion and its mutants.PrPsc was changed to PrPc through conformational change.PrP three-dimensional structures from different species have a similar globular domain from the 125 to 228 residues and a N-terminal flexibly disordered “tail”.The globular domain contains three alpha-helices comprising the residues 144-154,173-194,200-228 and a short anti-parallel beta-sheet comprising the residues 128-131 and 161-164,but they were different in the structure and surface charge distribution. |
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Key words: prion protein;conformation;property |
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