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鸡体内鹅源新城疫病毒抗原的免疫组织化学检测
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吴力力1，陈立功2，王小波1，崔华敏2，万洪全1*
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（1.扬州大学 兽医学院，江苏 扬州225009；
2.河北农业大学 动物科技学院，河北 保定071001）
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摘要：
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为研究鹅新城疫（ND）的发病机理，用3株鹅新城疫病毒强毒株分别人工感染25日龄雏鸡，以单克隆抗体介导的免疫组化（IHC）法检测攻毒鸡体内NDV抗原的分布及定位，研究了鹅新城疫病毒（NDV）对鸡的组织嗜性。结果显示，在试验鸡多种组织器官中均能检测到NDV抗原，病毒抗原主要定位于淋巴细胞、网状细胞、巨噬细胞、肝细胞及各种上皮细胞的胞浆内。结果表明，鹅NDV强毒株对鸡是一种泛嗜性病毒。 |
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关键词：
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新城疫病毒；鹅；组织嗜性；免疫组化法 |
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中图分类号：S 852.659.5 文献标识码：
A 文章编号：1673-4696(2008)01-0006-04 |
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Detection of antigen of Newcastle disease virus of goose origin in
chickens by immunohistochemical staining |
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WU Li-li1,CHEN Li-gong2,WANG
Xiao-bo1,CUI Hua-min2,WAN Hong-quan1 |
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(1.College of Veterinary Medicine,Yangzhou University,Yangzhou 225009,China；
2.College of Animal Science and Technology,Hebei Agricultural University,Baoding 071001,China) |
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Abstract: The tissue tropism of Newcastle disease virus （NDV）of goose origin to chickens was exa-mined to elucidate the pathogenesis of goose Newcastle disease（ND）. Twenty-five day-old chickens were inoculated with 3 velogenic NDVs isolated from goose flocks with clinical symptomes of ND. The location and distribution of the viral antigen in tissues from the inoculated chickens were examined by using immunohistochemical (IHC) staining mediated with monoclonal antibody against the NDV F protein. The viral antigen was detected in some organs and tissues, and located in the cytoplasm of lymphocytes, reticulocytes, macrophages, hepatocytes and various epithelial cells. These results showed that the 3 velogenic NDV isolates have pantropic tropism to chickens. |
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Key words: Newcastle disease virus;goose;tissue tropism;immunohistochemical staining |
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