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柔嫩艾美球虫免疫与鸡盲肠扁桃体TNF-α基因
表达动态的关系
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王彩霞，徐赓，王黎霞，安健*
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（北京农学院 动物科学技术系，北京102206）
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摘要：
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根据GenBank上鸡β-actin、TNF-α的基因序列设计引物，应用逆转录-聚合酶链式反应（RT-PCR）技术克隆获得了β-actin和TNF-α基因，采用β-actin为内参的半定量方法检测TNF-α mRNA在鸡柔嫩艾美球虫免疫前后不同时间的表达情况，以探讨TNF-α基因的表达动态与柔嫩艾美球虫免疫的关系。结果显示，TNF-α基因在两次免疫期间的表达量整体上呈现双峰模式，首免后第9d达到一个高峰，二免后第7d达到另一个高峰。结果表明，TNF-α在抗球虫感染中有一定的作用。 |
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关键词：
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β-actin基因；柔嫩艾美球虫；盲肠扁桃体；RT-PCR； TNF-α基因 |
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中图分类号：S 852.723 文献标识码：
A 文章编号：1673-4696(2008)01-0020-05 |
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Relationship between Eimeria tenella immunity and kinetic
expression of TNF-α gene in chicken cecal tonsil |
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WANG Cai-xia,XU Geng,WANG Li-xia,AN Jian
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(Department of Animal Science and Technology,Beijing College of Agriculture,Beijing 102206,China) |
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Abstract: According to chicken β-actin and TNF-α gene sequences in GenBank, primers were designed, and the gene sequences were amplified by reverse transcriptase-polymerase chain reaction(RT-PCR) from the cecal tonsil of chicken experimentally immunized with Eimeria tenella. The expression le-vel of TNF-α mRNA at different time after immunization was detected by semi-quantitative RT-PCR with β-actin gene as internal reference to study the relationship between E.tenella immunization and the kinetic expression of TNF-α gene in chicken cecal tonsil. The results showed that the level of expression of TNF-α gene reached 1st peak on day 9 post-1st-immunization, and reached 2nd peak on day 7 post-booster-immunization. It was concluded that TNF-α had some effect against E.tenella infection. |
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Key words: β-actin gene；Eimeria tenella;cecal tonsil；RT-PCR；tumor necrosis factor-α gene |
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