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德州足螨副肌球蛋白基因的克隆与序列分析
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郑晶，张晓谦，杨光友*，古小彬，余增莹
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（四川农业大学 动物医学院，四川 雅安625014）
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摘要：
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根据GenBank中登录的绵羊痒螨副肌球蛋白基因序列（登录号为AM114275）设计了3对引物，采用RT-PCR法从德州足螨总RNA中克隆了副肌球蛋白基因，研究了该基因与部分螨类副肌球蛋白基因的同源性以及推导的氨基酸序列之间的同源性。结果显示，该基因全长2628bp，编码875个氨基酸，预测分子质量约为97.24ku。与已报道的绵羊痒螨、欧洲尘螨、美洲尘螨、人疥螨和热带无爪螨的副肌球蛋白基因同源性分别为95.5%、90.5%、89.4%、82.6%和79.5%，推导的氨基酸同源性分别为98.0%、97.0%、980%、94.0%和89.0%。 |
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关键词：
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德州足螨；副肌球蛋白基因；克隆；序列分析 |
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中图分类号：S 852.746：Q 785
文献标识码：
A 文章编号：1673-4696(2008)01-0038-04 |
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Cloning and sequence analysis of Chorioptes texanus paramyosin gene |
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ZHENG Jing，ZHANG Xiao-qian，YANG Guang-you，GU Xiao-bin，YU Zeng-ying |
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（College of Veterinary Medicine,Sichuan Agricultural University，Ya’an 625014,China） |
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Abstract: Three pairs of specific primers for Chorioptes texanus paramyosin gene were designed according to the published sequence of Psoroptes ovis paramyosin gene （Accession number AM114275） in GenBank.The open reading frame (ORF) of the complete paramyosin gene was amplified by RT-PCR from C.texanus total RNA.The sequence identities of the amplified gene and its deduced amino acid were compared with those of some mites.The results showed that the gene was 2628bp in length,encoding an 875aa and had 95.5%，90.5%，89.4%，82.6% and 79.5% identity with the paramyosin genes of P.ovis，Dermatophagoides pteronyssinus (Der p 11)， Dermatophagoides farinae (Der f 11)，Sarcoptes scabiei and Blomia tropicalis,respectively.The deduced amino acid had 98.0%，97.0%，98.0%，94.0% and 89.0% identity with the expressed paramyosins of P.ovis，Der p 11，Der f 11，S.scabie and B.tropicalis,respectively. |
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Key words: Chorioptes texanus；paramyosin gene；cloning;sequence analysis |
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