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母鼠妊娠期铅镉联合暴露对仔鼠脑组织的氧化损伤
及乙酰半胱氨酸的保护效应
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路浩1 ，达剑森1 ，梅莉2 ，张英1，王林1，卓丽玲1， 刘宗平1*
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（1.扬州大学 兽医学院，江苏 扬州225009；2.甘肃农业大学 动物医学院，甘肃 兰州730070）
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摘要：
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45只怀孕SD母鼠被随机分为9组，即A组(对照组，饮用蒸馏水）、B组(铅组，300mg/L）、C组\[铅+N-乙酰半胱氨酸(N-NAC)组，300mg/L+20mmol/L\]、D组(镉组，10mg/L）、E组(镉+NAC组，10mg/L+20mmol/L）、F组(低剂量铅镉联合组，150mg/L+5mg/L）、G组(低剂量铅镉联合+NAC组，150mg/L+5mg/L+20mmol/L）、H组(高剂量铅镉联合组，300mg/L+10mg/L）、I组(高剂量铅镉联合+NAC组，300mg/L+10mg/L+20mmol/L）。采用饮水染毒，染毒时间为21d，分娩后每窝随机选取6只乳鼠测定脑匀浆超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)、乙酰胆碱酯酶(TChE)活性和丙二醛(MDA)的含量。结果表明，低剂量铅镉联合作用具有中间效应（介于铅、镉单独毒性作用之间），而高剂量铅镉联合作用表现协同或叠加效应。NAC拮抗组与染毒组比较，MDA含量显著降低，SOD、GSH-Px、TChE、CAT活性有升高趋势，说明NAC可以提高大鼠的抗氧化能力，对母鼠铅镉暴露所致新生鼠脑脂质过氧化损伤有一定的保护作用。 |
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关键词：
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铅镉联合；中枢神经系统；脂质过氧化；乙酰半胱氨酸；抗氧化酶；中间效应；协同效应 |
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中图分类号：S 856.9 文献标识码：
A 文章编号：1673-4696(2008)01-0042-04 |
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Lipid peroxidation of combined exposure to lead and cadmium
and antagonism of N-acetyl cysteine in neonatal brain to pregnant rats |
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LU Hao1,DA Jian-sen1,MEI Li2,ZHANG
Ying1,WANG Lin1,ZHUO Li-ling1,LIU
Zong-ping1 |
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(1.College of Veterinary Medicine,Yangzhou University,Yangzhou 225009,China;
2.College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,China) |
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Abstract: Forty-five adult female SD rats were separated into control group,lead acetate group(300mg/L),lead acetate+N-acetyl cysteine(NAC)(300mg/L+20mmol/L) group,cadmium chloride group(10mg/L),cadmium chloride+NAC group(10mg/L+20mmol/L),group of low doses of lead and cadmium(150mg/L+5mg/L),group of low doses of lead and cadmium+NAC(150mg/L+5mg/L+20mmol/L),and group of high doses lead and cadmium(300mg/L+10mg/L),group of high doses of lead and cadmium+NAC(300mg/L+10mg/L+20mmol/L),and the additives were given in the drinking water.After gestational periods the activities of SOD,MDA,GSH-Px,TChE and CAT in brain were analyzed respectively.The results showed that low doses of lead and cadmium appeared the intermediate effects (between toxicity of single Pb and Cd),whereas high doses showed the additive effects.Compared the NAC antagonistic groups with the poisoning groups,activities of SOD,GSH-Px,TChE,and CAT increased to certain extent,but content of MDA significantly decreased.The results indicated that NAC could increase antioxidant effects and reduce lipid peroxidation in SD rats. |
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Key words: combined lead and cadmium;Central nervous system;lipid peroxidation;N-acetyl cysteine;antioxidase;intermediate effect;additive effect |
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