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抗氧化酶在硝基苯中毒小鼠睾丸细胞凋亡中的作用
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王兴庄，邢厚娟，徐世文*
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（东北农业大学 动物医学院,黑龙江 哈尔滨150030）
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摘要：
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将10周龄雄性昆明小鼠随机分为硝基苯染毒组（26mg/kg、52mg/kg、105mg/kg）、花生油溶剂对照组、生理盐水对照组。对各组试验小鼠进行处理后，于第30d检测小鼠血清和睾丸中SOD、GSH-Px、CAT的活性及MDA含量，并通过DNA Ladder条带和透射电镜检测睾丸细胞的凋亡。结果，染毒组血清及睾丸中SOD、GSH-Px、CAT的活性显著或极显著(P<0.01或P<0.05)低于溶剂对照组，MDA含量显著或极显著(P<0.01或P<0.05)高于溶剂对照组，染毒组均显示DNA Ladder条带；电镜下细胞核皱缩，异染色质边聚，呈现典型凋亡现象。结果表明，硝基苯中毒能够诱使睾丸发生氧化应激，进而导致睾丸细胞发生凋亡，抗氧化酶在睾丸细胞凋亡中具有一定的作用。 |
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关键词：
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硝基苯；睾丸细胞；抗氧化酶；凋亡；小鼠 |
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中图分类号：Q 954.4：Q 255 文献标识码：
A 文章编号：1673-4696(2008)01-0046-04 |
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Effect of antioxidase on testis cell apoptosis induced by
nitrobenzene in mice |
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WANG Xing-zhuang,XING Hou-juan,XU Shi-wen |
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（College of Veterinary Medicine,Northeast Agricultural University,Harbin 150030,China） |
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Abstract: Ten week old male Kunming mice were divided randomly into 3 nitrobenzene treated groups(26mg/kg,52mg/kg and 105mg/kg,respectively),arachis oil control group and normal saline control group. The activities of SOD, GSH-Px and CAT and the content of MDA in serum and testicle were exa-mined on day 30 post-treatment. Testis cell apoptosis was detected by DNA Ladder and transmission electron microscope. The activities of SOD,GSH-Px and CAT in the 3 nitrobenzene treated groups were significantly lower than those in the arachis oil control group,and the contents of MDA in the 3 nitrobenzene treated groups were significantly higher than that in the solvent control group(P<0.01 or P<0.05).DNA Ladder appeared in the 3 nitrobenzene treated groups.Typical apoptosis was observed by the electron microscope.The result showed that nitrobenzene could induce oxidative stress,and lead to the apoptosis of testis cell in male mice,and antioxidase played a great role in testis cell apoptosis of the male mice. |
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Key words: nitrobenzene;testis cell;antioxidase;apoptosis;mice |
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