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妊娠期绵羊子宫内膜Flt-1基因表达的
RT-PCR检测
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姜怀志1，戢爽1，2,高爱萍1，李向军1，栾维民1*
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（1.吉林农业大学 动物科技学院,吉林 长春130118；2.延边大学 农学院，吉林 龙井133400）
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摘要：
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以β-actin基因作为内源性内标，采用RT-PCR方法检测了Flt-1基因mRNA在东北细毛羊、小尾寒羊妊娠期不同阶段子宫内膜中的表达量。结果显示，绵羊妊娠期在不同阶段的子宫内膜中Flt-1 mRNA均强烈表达，且随着妊娠期的延长，Flt-1 mRNA的表达量有逐渐增强的趋势(P<0.05)；在相同的妊娠阶段小尾寒羊子宫内膜中的Flt-1 mRNA表达量显著地高于东北细毛羊的Flt-1 mRNA表达量(P<0.05)。 |
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关键词：
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Flt基因；子宫内膜；RT-PCR；妊娠期；绵羊 |
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中图分类号：Q 954.592：Q 786 文献标识码：
A 文章编号：1673-4696(2008)01-0050-04 |
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Detection of expression of fms-like tyrosine kinase gene in endometrium
of sheep during pregnancy by RT-PCR |
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JIANG Huai-zhi1,JI Shuang1,2,GAO
Ai-ping1,LI Xiang-jun1,LUAN Wei-min1 |
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(1.College of Animal Science and Technology,Jilin Agricultural University,Changchun 130118,China；
2.College of Agriculture,Yanbian University,Longjing 133400,China) |
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Abstract:With beta-actin gene as the internal standard, reverse transcription-polymerase chain reaction (RT-PCR) was used to examine the expression of fms-like tyrosine kinase(Flt-1) mRNA in endometrium of Small Tail Han sheep and Northeast Fine-wool sheep. The expression of the Flt-1 mRNA was strong during the whole pregnancy. The level of expression of Flt-1 gene became higher along with conti-nuance of pregnancy(P<0.05). Flt-1 mRNA level in endometrium of Small Tail Han sheep was significantly higher than that in Northeast Fine-wool sheep during the same pregnancy stages(P<0.05). |
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Key words: fms-like tyrosine kinase gene;endometrium；reverse transcription-polymerase chain reaction;pregnancy;sheep |
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