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高铜对雏鸭肝氧化状态及肝细胞凋亡的影响
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赵丽，崔恒敏*，杨帆，彭西，邓俊良
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（四川农业大学 动物医学院，四川 雅安625014）
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摘要：
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将360只1日龄天府肉鸭随机分为6组，分别以对照日粮（Cu 8mg/kg）和高铜日粮（Ⅰ组：Cu 100mg/kg；Ⅱ组：Cu 200mg/kg；Ⅲ组：Cu 400mg/kg；Ⅳ组：Cu 600mg/kg；Ⅴ组：Cu 800mg/kg）饲喂6周，研究日粮铜水平对雏鸭肝氧化状态及肝细胞凋亡的影响。结果显示，与对照组比较，Ⅳ组和Ⅴ组肝铜锌SOD和谷胱甘肽过氧化物酶活性降低（P<0.01），Ⅲ组、Ⅳ组和Ⅴ组羟自由基和丙二醛含量显著升高（P<0.01），Ⅰ组和Ⅱ组肝铜锌SOD和谷胱甘肽过氧化物酶活性升高（P<0.01）。高铜组肝细胞凋亡率随日粮铜水平的升高而增加。表明，日粮铜水平为400mg/kg以上时可引起肝抗氧化功能降低和肝细胞凋亡率升高。 |
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关键词：
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雏鸭；高铜；肝；氧化状态；肝细胞凋亡 |
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中图分类号：S 816.72 文献标识码：
A 文章编号：1673-4696(2008)01-0054-05 |
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Effects of high dietary copper on hepatic oxidation and
hepatocyte apoptosis in ducklings |
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ZHAO Li,CUI Heng-min,YANG Fan,PENG Xi,DENG Jun-liang |
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(College of Veterinary Medicine,Sichuan Agricultural University,Ya’an 625014,China) |
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Abstract:Three hundred and sixty Tianfu meat ducklings of one-day-old were randomly divided into 6 groups，60 each,and raised on diets as follows:control dietary group (Cu 8mg/kg) and high copper dietary group (100mg Cu per 1kg diet in Group Ⅰ,200mg Cu per 1kg diet in Group Ⅱ,400mg Cu per 1kg diet in Group Ⅲ,600mg Cu per 1kg diet in Group Ⅳ, and 800mg Cu per 1kg diet in GroupⅤ) for 6 weeks.The activities of hepatic copper-zinc superoxide dismutase and glutathione peroxidase were decreased significantly in Groups Ⅳ and Ⅴ compared with those of the control dietary group（P<0.01）,but increased significantly in Groups Ⅰand Ⅱ（P<0.01）.The contents of hydroxy radical and malondialdehyde in livers increased significantly in Groups Ⅲ,Ⅳ and Ⅴ（P<0.01）.The hepatocyte apoptotic rates in the high copper dietary groups increased with the increase of copper level in diet.The results showed that dietary copper in excess of 400mg/kg impaired liver antioxidant function and induced hepatocyte apoptosis. |
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Key words: duckling;high copper;liver;oxidation;hepatocyte apoptosis |
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