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新城疫病毒F基因重组鸡痘病毒的安全性及免疫效力
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张体银1,2，王光锋1，孙蕾1，梁萍1，刘秀梵1*
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(1.扬州大学 农业部畜禽传染病学重点开放实验室，江苏 扬州225009；
2.福建出入境检验检疫局，福建 福州350001）
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摘要：
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将重组鸡痘病毒rFPVLP-SF分别皮下接种1日龄SPF鸡和26日龄商品鸡,评价其安全性和免疫效力。SPF鸡接种后除282E4株鸡痘病毒组在接种部位出现痘斑外，其他组鸡无任何临床症状。抗体检测结果表明，免疫接种后rFPVLP-SF组鸡的血清抗体水平没有显著变化，免疫后第21d以1×105ELD50的F48E8株NDV滴鼻攻毒，观察14d，rFPVLP-SF和油乳剂灭活疫苗免疫鸡的保护率分别为92%和100%，二者没有显著差异；大空斑株鸡痘病毒组和攻毒对照组鸡均全部死亡。结果显示，该重组病毒具有良好的安全性和免疫效力，有一定的应用前景。 |
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关键词：
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新城疫病毒；F基因；重组鸡痘病毒；安全性；免疫效力 |
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中图分类号：S 852.659.5 文献标识码：
A 文章编号：1673-4696(2008)02-0116-04 |
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Safety and immune efficacy of recombinant fowlpox virus expressing
fusion protein of Newcastle disease virus |
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ZHANG Ti-yin1,2,WANG Guang-feng1,SUN
Lei1,LIANG Ping1,LIU Xiu-fan1 |
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(1.Key Laboratory of Animal Infectious Disease of the Ministry of Agriculture/Yangzhou University,Yangzhou
225009,China;2.Fujian Entry-Exit Inspection and Quarantine Bureau,Fuzhou 350001,China) |
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Abstract: One-day-old SPF chickens(4 groups of 8 each) or 26-day-old commercial layers(4 groups of 25 each) were immunized,respectively with the recombinant fowlpox virus(rFPVLP-SF) expressing fusion protein of ZJ-1 strain of Newcastle disease virus(NDV) by subcutaneous route at the dosage of 1×105PFU in order to evaluate,respectively,its safety and immune efficacy.With the exception of the SPF chickens inoculated with FPV strain 282E4,the SPF chickens had no any clinical symptoms.Detection of a antibody indicated that serum antibodies level to rFPVLp-SF of NDV slightly changed after inoculation in the chickens and all commercial chickens were challenged intranasally with 1×105ELD50 of F48E8 strain NDV on day 21 after vaccination.The protection rates of rFPVLp-SF and inactivated vaccine in oil emulsion were 92% and 100%,respectively,without significant difference(P>0.05),while all of the chickens vaccinated with Large plaque strain of Fowlpox virus(FPVLP) and unvaccinated died.The results showed that rFPVLP-SF was safe and effective for protecting chickens from ND.
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Key words: Newcastle disease virus;fusion protein gene;recombinant fowlpox virus;safety;immune efficacy |
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