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乳牛衣原体病灭活疫苗效检小鼠模型的建立
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邱昌庆，周继章，程淑敏，曹小安,蔺国珍
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（中国农业科学院 兰州兽医研究所 家畜疫病病原生物学国家重点实验室，甘肃 兰州730046）
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摘要：
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为了解决以本动物效检乳牛衣原体病灭活疫苗成本高的问题，用11批乳牛衣原体病灭活疫苗做乳牛效力试验时同步做小鼠效力试验，除第3批次疫苗的本动物效检结果与小鼠效检结果略有差异外，其他批次疫苗的效检结果均一致。结果表明，用小鼠做牛衣原体灭活疫苗的效检动物模型是可行的。 |
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关键词：
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乳牛；衣原体病灭活疫苗；效力试验；小鼠模型 |
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中图分类号：S 852.67
文献标识码：
A 文章编号：1673-4696(2008)02-0120-03 |
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Establishment of mouse model for potency determination
of the inactivated vaccine against chlamydiosis in cow |
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QIU Chang-qing,ZHOU Ji-zhang,CHENG Shu-min,CAO Xiao-an，LIN Guo-zhen
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(State Key Laboratory of Veterinary Etiological Biology/Lanzhou Veterinary Research Institute,
Chinese Academy of Agricultural Sciences,Lanzhou 730046,China) |
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Abstract: In order to reduce high cost of potency test for the inactivated vaccine against dairy cow chlamydiosis,target animal dairy cows and mice were inoculated with various doses of 11 batches of the inactivated vaccines,respectively,and then they were challenged with Chlamydia psittaci virulent strain SX5 on day 21 post-vaccination.The results showed that 10 of 11 batches of the target animal potency tests were consistent with mice potency tests,with the exception of the batch 3 in which there was very little difference.So it is feasible to use mice for assaying potency of the inactivated vaccine against dairy cow chlamydiosis,thus replacing target animal dairy cow. |
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Key words: cow;inactivated vaccine against chlamydiosis;potency test;mouse model |
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