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三氧化二砷对鸡马立克氏病肿瘤细胞凋亡的影响
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张久丽1,2，李忠显2，吴立君2，徐世文1*
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（1．东北农业大学 动物医学院，黑龙江 哈尔滨150030；
2．黑龙江畜牧兽医职业学院，黑龙江 双城150100）
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摘要：
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研究了三氧化二砷（As2O3）对诱导鸡马立克氏病(MD)肿瘤细胞株MDCC-MSB1细胞凋亡相关基因Fas、Caspase-3 mRNA表达及Caspase-3活性的影响，进而探讨了As2O3诱导MD肿瘤细胞株凋亡的机制。以体外培养MDCC-MSB1为研究对象，经终浓度分别为0、2、4和8μmol/L的As2O3作用48h后，采用荧光显微镜观察细胞的形态学变化，DNA Ladder法检测细胞凋亡情况，RT-PCR方法检测Fas、Caspase-3 mRNA的表达水平，试剂盒比色法检测Caspase-3活性的变化。结果表明，As2O3能引起典型的凋亡形态学变化，并出现典型的DNA Ladder，同时Fas、Caspase-3 mRNA的表达水平和Caspase-3活性均增加，组间差异均极显著(P<0.01)，呈现剂量依赖性。证实As2O3诱导鸡MD肿瘤细胞株MDCC-MSB1细胞凋亡是通过增加Fas、Caspase-3 mRNA的表达和提高Caspase-3活性来实现的。 |
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关键词：
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三氧化二砷；MDCC-MSB1；细胞凋亡；Fas；Caspase-3 |
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中图分类号：S 852.659.6：Q 503 文献标识码：
A 文章编号：1673-4696(2008)02-0137-05 |
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Effect of arsenic trioxide on apoptosis of cancer cells from chicken
with Marek’s disease |
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ZHANG Jiu-li1,2，LI Zhong-xian2，WU
Li-jun2，XU Shi-wen1* |
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(1.College of Veterinary Medicine,Northeast Agricultural University,Harbin 150030,China；
2.Heilongjiang Vocational College of Animal Husbandry and Veterinary Science,Shuangcheng 150100,China) |
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Abstract: Effect of arsenic trioxide(As2O3) on the expression of Fas and Caspase-3 mRNA and on the activation of Caspase-3 in Marek’s disease(MD) cancer cell line(MDCC-MSB1) was studied in vitro to explore its mechanisms. MDCC-MSB1 cells were treated respectively with As2O3 at 0,2,4,8μmol/L. At 48th h post-treatment, morphological changes were examined by fluorescence microscope,apoptosis was examined by DNA Ladder,and the expressions of Fas and Caspase-3 mRNAs and the activity of Caspase-3 were measured by RT-PCR and Caspase-3 Colorimetric Assay Kit. Typical apoptosis characteristics was observed by fluorescence microscope.The expressions of Fas,Caspase-3 mRNA and the activity of Caspase-3 were increased(P<0.01) after treatment with As2O3 in a concentration dependent manner. The results showed that As2O3 induced apoptosis of MDCC-MSB1 cells in vitro through the increase of expressions of Fas and Caspase-3 mRNAs and through enhancing activity of Caspase-3. |
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Key words: As2O3;MDCC-MSB1;apoptosis;Fas;Caspase-3 |
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