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高铜日粮对肉鸡肝细胞线粒体呼吸链复合物
活性的影响
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曹华斌1，2，李和平1，苏荣胜1，郭剑英1，李成梅1，唐兆新1*
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(1.华南农业大学 兽医学院，广东 广州510642； 2.江西农业大学 动物科技学院，江西 南昌330045)
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摘要：
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在对照组(Cu 11mg/kg，A组)基础上设高铜组3个（110mg/kg、220mg/kg和330mg/kg，分别对应为试验组B、C、D），试验期为60d，在12、24、36、48、60日龄采集肝组织，提取线粒体，测定线粒体复合物Ⅰ、Ⅱ、Ⅲ、Ⅳ和Ⅴ的活性变化。结果表明，11mg/kg和110mg/kg铜日粮添加组线粒体复合物Ⅰ、Ⅱ、Ⅲ、Ⅳ、Ⅴ的活性呈增加趋势。220mg/kg和330mg/kg铜日粮添加组肉鸡肝细胞线粒体复合物Ⅰ、Ⅱ、Ⅲ、Ⅳ、Ⅴ活性减弱，但主要在试验后期，尤其是48日龄之后，比对照组和各组内的试验前期下降明显（P<0.05）。说明高铜日粮在一定程度上对线粒体复合物活性是有利的，但过高的铜添加到日粮中（≥220mg/kg）可降低肉鸡肝细胞线粒体呼吸链复合物的功能活性。据此可以推测，线粒体是机体铜过量状态下侵害的靶部位之一，而且能作用于线粒体各个复合物，造成线粒体呼吸功能减弱，呼吸链中电子传递发生故障，从而影响线粒体功能的正常发挥。 |
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关键词：
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肉鸡；高铜日粮；线粒体复合物；活性 |
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中图分类号：S 816.72：Q 952.6 文献标识码：
A 文章编号：1673-4696(2008)02-0155-05 |
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Effect of high-copper diet on activities of respiratory chain complexes
in liver mitochondria of broilers |
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CAO Hua-bin1,2,LI He-ping1,SU
Rong-sheng1,GUO Jian-ying1,LI Cheng-mei1,TANG
Zhao-xin1 |
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(1.College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642,China;
2.College of Animal Science and Technology,Jiangxi Agricultural University,Nanchang 330045,China) |
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Abstract: In addition to the control group(Cu 11mg/kg，Group A),three experimental groups were set up,they were Groups 110mg/kg(Group B),220mg/kg(Group C) and 330mg/kg(Group D) respectively. All of the experimental broilers were fed with corn-soybean meal for 60 days, and the activities of mitochondrial complex from broilers' hepatocyte were assayed on 12,24,36,48 and 60 day-old. The high-copper diet had influence on liver mitochondrial complex,the activities of each complex in Groups A and B had a tendency of increasing, more obviously in Group B（P<0.05）,but were subject to decrease in Group C and D（P<005）,especially on the later stage of whole experiment（after 48 day-old）,which indicated that high-copper diet could be helpful to liver mitochondrial complex of broiler to some extent, but too high-copper diet (≥220mg/kg) might degrade activity of them. It was presumed that mitochondria was the invasive target under the condition of copper excess, and this effect was mainly on mitochondrial complex which resulted in decreasing of respiratory function and breakdown of electron transfer,then made seriously mitochondrial electron transport chain broke down,which may be was one of the essential reasons of copper toxicity to living organism.
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Key words: broiler;high-copper diet;mitochondrial complex;activity |
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