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头孢喹肟对大肠杆菌防突变浓度的测定
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杨大伟，陈杖榴，丁焕中*，冯宝仪，王贤玉，梁艳玲
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（华南农业大学 兽医学院 广东省兽药研制与安全评价重点实验室，广东 广州510642）
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摘要：
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采用标准琼脂二倍稀释法测定了头孢喹肟对大肠杆菌的最低抑菌浓度；用肉汤法富集了大肠杆菌；用琼脂稀释法测定了头孢喹肟对大肠杆菌的暂定防突变浓度（MPCpr）及防突变浓度（MPC）。结果显示，头孢喹肟对大肠杆菌标准株（ATCC25922）的MIC和MIC99分别为0.125μg/mL和0.1μg/mL；MPCpr和MPC分别为0.5μg/mL和0.256μg/mL，MPC/MIC99比值为2.56。头孢喹肟对50株大肠杆菌临床分离株的MIC90和MPC90分别为0.2μg/mL和0.8μg/mL,MPC90/MIC90比值为4。说明头孢喹肟限制大肠杆菌耐药突变的能力很强，ATCC25922的选择指数(SI)仅为2.56，临床分离株的SI为4。 |
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关键词：
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头孢喹肟；大肠杆菌；防突变浓度；选择指数 |
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中图分类号：S 859.796 文献标识码：
A 文章编号：1673-4696(2008)02-0165-04 |
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Determination of mutant prevention concentration of cefquinome
against Escherichia coli |
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YANG Da-wei,CHEN Zhang-liu,DING Huan-zhong,FENG Bao-yi,WANG Xian-yu,LIANG Yan-ling |
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（Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation/
College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642,China） |
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Abstract: The minimal inhibitory concentration(MIC) and MIC for 99%of input cells（MIC99）of cefquinome against Escherichia coli were determined by agar two-fold dilution method.The E.coli was enriched in broth,and the bacterial concentrations were adjusted to 1×1010 colony forming units(CFU) per milliliter.The provisional mutant prevention concentration(MPCpr) and mutant prevention concentration(MPC) of cefquinome against E.coli were determined by agar plates dilution method.The MIC,MIC99,MPCpr,MPC and MPC/MIC99 of cefquinome against E.coli strain ATCC25922 were 0.125μg/mL,0.1μg/mL,0.5μg/mL,0.256μg/mL and 2.56,respectively.The MPC for 90% of the isolates(MPC90) and the MPC90/MIC90 of cefquinome against 50 E.coli clinical isolates were 0.8μg/mL and 4,respectively.The results showed that the capacity of cefquinome for restricting the selection of E.coli resistant mutant was very strong and selection indexes of ATCC25922 strain and the clinical isolates were 2.56 and 4,respectively. |
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Key words: cefquinome;Escherichia coli;mutant prevention concentration(MPC);selection
index(SI) |
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