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兔圆小囊及肠道组织中抗菌肽的免疫组织化学和
免疫电镜细胞化学定位
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佘锐萍1，李冰玲2，刘伟1，刘玉如1
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(1.中国农业大学 动物医学院 黏膜免疫病理学实验室，北京100094；
2.北京出入境检验检疫局，北京100029）
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摘要：
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采用免疫组织化学和免疫电镜细胞化学技术观察了兔圆小囊及其他肠道组织中产抗菌肽细胞的分布定位。免疫组织化学观察发现，产抗菌肽细胞在兔圆小囊的黏膜上皮、圆顶上皮以及淋巴组织的滤泡生发中心、圆顶区和帽区均有分布；在兔感染多杀性巴氏杆菌后，圆小囊及其他肠道组织中的抗菌肽免疫组织化学阳性细胞数量明显增多，阳性反应增强。免疫电镜细胞化学观察结果显示，除肠道黏膜上皮细胞、异嗜性白细胞、巨噬细胞中存在抗菌肽免疫组织化学阳性反应信号外，在淋巴细胞内也发现阳性反应信号，尤其是在圆小囊淋巴组织中的DNES细胞内发现抗菌肽免疫组织化学阳性反应信号。表明圆小囊的DNES细胞和淋巴细胞均可产生抗菌肽。 |
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关键词：
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兔圆小囊；抗菌肽；DNES细胞；免疫组织化学；免疫电镜细胞化学 |
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中图分类号：S 852.165：Q 503 文献标识码：
A 文章编号：1673-4696(2008)03-0239-05 |
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Immunohistochemical and immunoelectron microscopic cytochemical
localization of the antibacterial peptides in rabbit
sacculus rotundus and intestine |
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SHE Rui-ping1,LI Bing-ling2,LIU
Wei1 ,LIU Yu-ru1 |
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(1.Department of Pathology and Mucosal Immunity,College of Veterinary Medicine,China Agricultural
University,Beijing 100094,China;2.Beijing Entry-Exit Inspection and Quarantine Bureau,Beijing 100029,China) |
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Abstract:The distribution of cells producing the antibacterial peptides(ABPs) in rabbit sacculus rotundus(RSR)(an immune-neuro-endocrine organ at the connection of ileum and caecum)and intestine were studied with immunohistochemistry and immunoelectron microscopic cytochemistry.Immunohistochemically the cells that were positive immunoreactivity of ABPs antigen distributed in villi epithelium and dome epithelium and in follicle,dome,and cap zone of the lymphoid tissue of RSR.Compared with those in control group，the number of positive immunoreactivity cell in RSR and intestine increased and its intensity of immunoreactivity enhanced obviously after rabbit was infected with Pasteurella multocida.The immunoelectron microscopic cytochemical observation showed that the positive immuno reactivity was found in epithelial cells,neutrophils,macrophages,lymphocytes and DNES(disperse or diffuse neuroendocrine system) cells.The present study demonstrated for the first time that ABPs were distributed in lymphocyte and DNES cell of the RSR. |
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Key words: rabbit sacculus rotundus;antibacterial peptide；DNES cells;immunohistochemistry;immunoelectron microscopic cytochemistry |
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