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iNOS基因和VEGF基因在氟致甲状腺肿大鼠
甲状腺组织中的表达
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张维东1，2，柴春彦1，张勇2，江鹏3，刘国艳1*
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（1.上海交通大学 农业与生物学院，上海200240；2.甘肃农业大学 动物医学院，甘肃 兰州730070；
3.华中农业大学 动物医学院，湖北 武汉430070）
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摘要：
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为探讨氟致甲状腺肿大的分子机理，选用4周龄SD大鼠饮用不同浓度的NaF水溶液，复制了氟中毒致大鼠甲状腺肿模型，光镜下观察了甲状腺的形态结构变化，并用RT-PCR技术检测了甲状腺组织中iNOS mRNA和VEGF mRNA的表达情况。结果，氟中毒大鼠甲状腺微血管明显扩张、增生，表现为明显的结节性甲状腺肿；与正常对照组大鼠相比，氟中毒组大鼠甲状腺组织中iNOS mRNA和VEGF mRNA的表达水平显著升高（P<0.05）。结果表明，长期摄入过量氟导致甲状腺发生结节性肿大的机理之一是，氟可刺激甲状腺中iNOS基因和VEGF基因的过量表达，进而造成甲状腺组织内微血管增生。 |
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关键词：
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氟；甲状腺肿；诱导型一氧化氮合成酶基因；血管内皮生长因子基因 |
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中图分类号：S 859.87 文献标识码：
A 文章编号：1673-4696(2008)03-0257-04 |
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Expressions of iNOS and VEGF mRNAs in thyroid gland of
rat with goiter induced by fluoride |
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ZHANG Wei-dong1,2,CHAI Chun-yan1,ZHANG
Yong2,JIANG Peng3,LIU Guo-yan1 |
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(1.College of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China;
2.College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,China;
3.College of Veterinary Medicine,Huazhong Agricultural University,Wuhan 430070,China) |
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Abstract: To investigate the mechanism of goiter caused by fluoride,goiter model of SD rats was produced by administering sodium fluoride in drinking water.Histological section of thyroid gland was made,and inducible nitric oxide synthase(iNOS) and vessel endothelial growth factor( VEGF) were determined by RT-PCR.Results showed that,the capillary vessels in thyroid glands of the rats treated with fluoride proliferated and an obvious nodular goiter occurred in the fluoride-treated rats.Compared with the control,the contents of iNOS and VEGF in the thyroid glands of the rats with fluorosis was increased significantly（P<0.05）.It was concluded from the results that the mechanism of goiter caused by fluoride was that fluoride induced the over-expressions of iNOS and VEGF mRNAs in thyroid gland,which caused hyperplasia of capillary vessels. |
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Key words: fluoride;goiter;iNOS gene；VEGF gene |
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