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人工感染仔猪猪生殖与呼吸综合征病毒的
核酸分布规律
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李雪梅1，2，3,程安春1，2*， 汪铭书1，2*
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（1.四川农业大学 动物医学院，四川 雅安625014;2.动物疫病与人类健康四川省重点实验室，四川 雅安
625014;3.宜宾职业技术学院，四川 宜宾644003）
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摘要：
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根据猪生殖与呼吸综合征病毒(PRRSV)美洲株ATCC VR-2332保守区ORF6和ORF7的基因序列，设计了1条37bp的寡核苷酸探针，末端用生物素标记。应用原位杂交方法对人工感染PRRSV SC-1株的28日龄仔猪进行了病毒核酸分布规律的研究。结果显示，感染后7～28d于肝、脾、肺、肾、扁桃体、胸腺、肺门淋巴结、肠和大脑检测到阳性杂交信号，其中肺门淋巴结的阳性信号一直较强，脾的阳性信号逐渐增强，肺的阳性信号较弱，心脏未见阳性杂交信号。 |
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关键词：
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猪生殖与呼吸综合征病毒；人工感染仔猪；原位杂交；分布 |
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中图分类号：S 852.659.6 文献标识码：
A 文章编号：1673-4696(2008)04-0278-05 |
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Nucleotide distribution of porcine reproductive and respiratory
syndrome virus in piglets infected experimentally with the virus |
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LI Xue-mei1,2,3,CHENG An-chun1,2,WANG
Ming-shu1,2 |
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(1.College of Veterinary Medicine，Sichuan Agricultural University,Ya’an 625014,China;
2.Key Laboratory of Animal Disease and Human Health of Sichuan Province,Ya’an 625014,China；
3.Yibin Vocational & Technical College,Yibin 644003,China) |
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Abstract: According to the conserve ORF6 and ORF7 gene sequences of the North American strain ATCC VR-2332 of porcine reproductive and respiratory syndrome virus(PRRSV)，a 37bp oligonucleotide probe was designed,and labeled with biotin.The distributions of PRRSV in the 28-day-old-piglets at diffe-rent time points post-infection were analyzed by using in situ hybridization.The result showed that positive signals were found from day 7 to 28 post-infection in tonsil,hilar node,thymus,spleen,lung,kidney,and cerebrum.Strong positive in situ hybridization signal was found in hilar node.The positive signals in spleen increased gradually post-infection.The weak positive signals were found in lung.Signal in heart was negative. |
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Key words: porcine reproductive and respiratory syndrome virus;piglet infected experimentally;in situ hybridization;distribution
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