|
|
|
食蟹猴和猕猴朊蛋白基因的克隆与序列分析
|
|
|
|
宁章勇，于博，邓衔柏，李玉谷，马勇江，孔小明
|
|
|
|
(华南农业大学 兽医学院，广东 广州510642)
|
|
|
|
摘要：
|
|
以外周血液的基因组DNA为模板，运用PCR方法扩增了食蟹猴和猕猴的朊蛋白基因,并将其克隆到pGEM-T Easy载体中进行测序，运用分子生物学软件对这些序列进行了分析。结果表明，所克隆的食蟹猴和猕猴朊蛋白基因的开放阅读框片段包含了朊蛋白基因的完整编码区，含有762个核苷酸,该基因内无内含子，编码253个氨基酸的前体蛋白，推测其分子质量约27.6ku 。与已报道的其他多种动物朊蛋白基因序列做比较，发现其与多种哺乳动物都有较高的同源性，其中与人的同源性最高。氨基酸点突变分析除发现了已报道的多态性位点N97S 、H100N外，在食蟹猴和猕猴均发现了未报道的S242L点突变位点。此外，在食蟹猴还发现了I8M、C151R点突变位点。 |
|
关键词：
|
|
食蟹猴；猕猴；朊蛋白；序列分析 |
|
中图分类号：S 852.659.7：Q 785 文献标识码：
A 文章编号：1673-4696(2008)04-0283-05 |
|
|
Cloning and sequence analysis of the prion protein genes of
cynomolgus monkey and rhesus monkey |
|
|
|
NING Zhang-yong,YU Bo,DENG Xian-bo,LI Yu-gu,MA Yong-jiang,KONG Xiao-ming |
|
|
|
(College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642,China) |
|
|
Abstract: Prion protein(PrP) genes of cynomolgus monkey and rhesus monkey were amplified respectively by PCR from the genomic DNAs extracted from the peripheral blood of the two species of animals as templates.The genes were sequenced after being cloned respectively into the vector pGEM-T Easy and analyzed with bioinformatics softwares.The cloned open reading frame(ORF) of prion protein genes of cynomolgus monkey and rhesus monkey consisted of 762 nucleotides and contained the entire prion protein gene coding sequence without intron.The coded precursor proteins with 253 amino acids were 27.6ku in mole-cular mass.The genes shared high identities with those of various mammals and the highest identity was observed with that of human.The mutation sites N97S and H100N(which had been reported previously),and S242L(which was not reported previously) were found in both cynomolgus monkey and rhesus monkey,whereas mutation sites I8M and C151R were only found in cynomolgus monkey. |
|
|
|
Key words: cynomolgus monkey;rhesus monkey;prion protein;sequence analysis |
|
|
|
|
|
《中国兽医科学》 ©
版权所有
Chinese Veterinary Science
zgsykx@zgsykx.com |
