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猪瘟病毒石门株与兔化弱毒疫苗株之间抗原
差异的单克隆抗体分析
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朱妍1，2,李素1,王文成3 ,涂长春2*
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(1.吉林大学 畜牧兽医学院，吉林 长春130062;2.军事医学科学院 军事兽医研究所，吉林 长春130062;
3.辽宁益康生物制品有限公司，辽宁 辽阳111000)
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摘要：
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采用间接免疫荧光方法，用19株抗猪瘟病毒单克隆抗体对猪瘟病毒石门株与疫苗株（兔化弱毒株）的抗原性进行分析。结果显示，9株抗猪瘟病毒E2蛋白的单抗中有4株与2株病毒均反应，但反应程度略有差别；9株抗猪瘟病毒Erns蛋白的单抗中只有3株与2株病毒都反应，其他6株在识别石门株与兔化弱毒株之间存在差别；抗猪瘟病毒NS2/3蛋白的单抗只识别弱毒株。表明，抗猪瘟病毒单抗可用于猪瘟病毒石门株与兔化弱毒疫苗株抗原性差异的分析。 |
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关键词：
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猪瘟病毒；单克隆抗体；抗原性分析 |
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中图分类号：S 852.659.6 文献标识码：
A 文章编号：1673-4696(2008)04-0293-04 |
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Analysis of antigenic difference between classical swine fever virus
Shimen strain and C-strain using monoclonal antibodies |
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ZHU Yan1,2,LI Su1,WANG Wen-cheng3,TU
Chang-chun2
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(1.College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China;
2.Institute of Military Veterinary Sciences,The Academy of Military Medical Sciences,Changchun 130062,China;
3.Liaoning Yikang Biological Product Co.,Ltd,Liaoyang 111000,China) |
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Abstract: Antigenicities of the classical swine fever virus(CSFV) Shimen strain and C-strain were analyzed with 19 monoclonal antibodies(McAbs) against CSFV by the indirect immunofluorescence assay.Four of 9 strains of McAbs against CSFV E2 protein could react differentially with the two strains of CSFVs.Three of 9 strains of McAbs against CSFV Erns protein could react with the two strains of CSFVs,and the other six could react differentially with the two strains of CSFVs.The McAbs against CSFV NS2/3 protein could only react with the C-strain.The results indicated that the McAbs against CSFV could be used to analyze the antigenic difference between the two strains of CSFVs. |
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Key words: classical swine fever virus;monoclonal antibody;antigenic analysis |
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