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4种终末宿主土耳其斯坦东毕吸虫cox1和nad1
基因的序列分析
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李利1，邢继兰2，王春仁1*，翟延庆1，何国声3
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（1.黑龙江八一农垦大学 动物科技学院，黑龙江 大庆163319；2.上海市农业科学院 畜牧兽医研究所，
上海201106；3.中国农业科学院 上海兽医研究所，上海200232）
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摘要：
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收集黑龙江省大庆地区牛源、绵羊源、绒山羊源和山羊源土耳其斯坦东毕吸虫，以SDS-蛋白酶K法抽提其基因组DNA，用特异性引物通过PCR扩增土耳其斯坦东毕吸虫成虫细胞色素C氧化酶亚基1基因(cox1)和烟酰胺腺嘌呤二核苷酸脱氢酶亚基1基因(nad1)并进行测序，应用Chromas和DNAStar软件分析扩增产物的变异情况。结果表明，大庆地区4种终末宿主土耳其斯坦东毕吸虫cox1基因均为1125bp，nad1基因均为518bp；且其线粒体基因存在差异，cox1的同源性为99.0%～99.7%，nad1的同源性为983%～99.4%。 |
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关键词：
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土耳其斯坦东毕吸虫；细胞色素C氧化酶亚基1基因；烟酰胺腺嘌呤二核苷酸脱氢酶亚基1基因；序列分析 |
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中图分类号：S 852.735
文献标识码：
A 文章编号：1673-4696(2008)04-0303-05 |
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Sequence analysis of cox1 and nad1 genes of Orientobilharzia
turkestanicum from 4 definitive hosts |
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LI Li1,XING Ji-lan2,WANG Chun-ren1,ZHAI
Yan-qing1,HE Guo-sheng3
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(1.College of Animal Science and Technology,Heilongjiang August-First Land Reclamation University,Daqing
163319,China;2.Institute of Animal Science and Veterinary Medicine,Shanghai Academy of Agricultural
Sciences,Shanghai 201106,China;3.Shanghai Veterinary Research Institute,Chinese Academy of
Agricultural Sciences,Shanghai 200232,China) |
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Abstract: Orientobilharzia turkestanicum were isolated from cattle,sheep,cashmere goat and native goat in Daqing Prefecture of Heilongjiang Province,China,and their genomic DNAs were extracted by SDS-protease K method.The cox1 and nad1 genes were amplified by PCR,sequenced,and analyzed by Chromas and DNAStar softwares.The results showed that the lengths of cox1 and nad1 sequences from the 4 definitive hosts of O.turkestanicum were 1125bp and 518bp,respectively.The homology of cox1 gene was from 990% to 99.7% and homology of nad1 gene was from 98.3% to 99.4% among the 4 definitive hosts. |
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Key words: Orientobilharzia turkestanicum;cox1 gene; nad1 gene;sequence analysis |
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