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JA1对体外培养的Hep细胞和小鼠皮下移植性
Hep细胞凋亡的影响
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贾宁1，方梅1，薛妍2
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（1.甘肃农业大学 动物医学院，甘肃 兰州730070；2.兰州铁路第四中学，甘肃 兰州730000）
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摘要：
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采用噻唑蓝（MTT）还原法、流式细胞术、光镜及透射电子显微镜技术等检测了梯度浓度的JA1(沙冬青提取物)对体外培养的小鼠肝癌细胞株Hep增殖的影响及诱导Hep细胞凋亡的作用；并检测了JA1对小鼠皮下移植性Hep细胞的生长抑制及诱导凋亡作用。结果显示，JA1可显著抑制小鼠Hep细胞的增殖，且这种抑制有浓度和时间依赖性。流式细胞仪分析显示，JA1处理后的Hep检测样本中有明显的DNA低含量颗粒（“亚G1期”峰），细胞周期各时相分布发生了改变，细胞在G1期被阻滞。同时，JA1对小鼠皮下移植性Hep细胞也有明显的抑制作用。JA1灌胃试验组瘤组织细胞DNA直方图有明显的凋亡峰，细胞在G1期也被阻滞，光镜和电镜下可见大量凋亡肿瘤细胞。 |
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关键词：
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沙冬青提取物；小鼠；Hep细胞；凋亡 |
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中图分类号：S 857.4：Q 952 文献标识码：
A 文章编号：1673-4696(2008)04-0332-06 |
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Apoptosis of Hep cells induced by JA1 in vitro and in vivo |
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JIA Ning1,FANG Mei1,XUE Yan2 |
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(1.College of Veterinary Medicine,Gansu Agricultural University，Lanzhou 730070,China;
2.No.4 Middle School of Lanzhou Railway Bureau,Lanzhou 730000,China) |
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Abstract:To investigate the inhibitory effect of the extractive of Ammopiptanthus mongolicus（JA1）on hepatocarcinoma cells and its possible mechanisms,the hepatocellular carcinoma cell line of mice（Hep）was used as target cell.The inhibitory effects of JA1 on Hep cells were assayed by MTT,apoptosis were mea-sured by flow cytometry in vitro and the antitumor effect of JA1 was also observed in Hep-bearing mice by flow cytometry assay,light microscopy and transmission electronic microscopy.JA1 had significant anti-proliferative effects on Hep cells with dependence of time and dosage.In addition,JA1 not only could inhi-bit significantly the proliferation of Hep cells in vitro but also inhibit the growth of Hep hepatocarcinoma in vivo.Flow cytometric analysis indicated that JA1 could induce apoptosis on Hep cells in Hep-bearing mice and the G1 phase cell percentage was incrassation and the S phase cell percentage decrease.Morphological change of Hep cells undergoing apoptosis were also detected by light and electron microscopies.These result showed that JA1 had good inhibitory effects on Hep in vitro and in vivo and the mechanism was related to its apoptosis inducing activity. |
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Key words: JA1(the extractive of Ammopiptanthus mongolicus)；mice；hepatocellular carcinoma cell line of mice（Hep）；apoptosis |
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