| |
|
 |
|
|
|
鸡盲肠扁桃体中T淋巴细胞及其亚群的发育
|
|
|
|
栾维民，杨树宝，杨丽华，张延林，高晶晶，何昭阳*
|
|
|
|
（吉林农业大学 动物科学技术学院,吉林 长春130118）
|
|
|
|
摘要：
|
|
通过免疫组织化学方法，应用CD3、CD4和CD8单克隆抗体研究了CD3+ T淋巴细胞及CD4+和CD8+T淋巴细胞亚群在鸡盲肠扁桃体中的出现、迁移、组织定位分布及数量变化规律等一系列发育过程。结果显示，CD3+、CD8+ T淋巴细胞最初于20日龄胚胎中出现，而CD4+ T淋巴细胞在出壳后1日龄时出现。在定位分布变化上，4日龄之前，T淋巴细胞主要分布在黏膜层的中下部区域，而4日龄以后的各日龄在整个黏膜层中均匀分布，CD4+细胞主要聚集在滤泡间区域，而CD3+细胞和CD8+细胞在固有层中和黏膜上皮中都有大量分布。在数量变化上，4日龄时，各种阳性细胞的数量都骤然增加，而到7日龄时，CD3+、CD8+T淋巴细胞的数量明显减少，CD4+细胞的数量无明显变化，从14日龄开始直到35日龄，CD3+、CD4+和CD8+ T淋巴细胞的数量持续增加。证实，鸡出壳后初期盲肠扁桃体的细胞免疫功能增强，并在14日龄时达到成熟水平。 |
|
关键词：
|
|
鸡；盲肠扁桃体；T淋巴细胞；亚群；发育 |
|
中图分类号：S 852.165：Q 503 文献标识码：
A 文章编号：1673-4696(2008)04-0338-04 |
|
|
Development of T lymphocyte subpopulations in caecal tonsil of chicken |
|
|
|
LUAN Wei-min,YANG Shu-bao,YANG Li-hua,ZHANG Yan-lin,GAO Jing-jing,HE Zhao-yang |
|
|
|
(College of Animal Science and Technology,Jilin Agricultural University,Changchun 130118,China)
|
|
|
Abstract: Occurrence,migration,tissue localization and quantity changes of CD3+,CD4+,and CD8+ T lymphocyte subpopulations in chicken caecal tonsil was examined immunohistochemically by using the CD3,CD4 and CD8 monoclonal antibodies.The findings showed that both CD3+and CD8+ T lymphocytes appeared firstly in caecal tonsil of embryo of 20-day-old and CD4+ cells appeared in 1-day-old broiler.T lymphocytes were distributed mainly in the middle and underneath of mucous layer before 4-day-old,but they distributed uniformly in the whole mucous layer after 4-day-old,CD4+ cells concentrated in the interfollicular areas,and both CD3+ and CD8+T lymphocytes distributed in the lamina propria and epithelium.Various kinds of positive cells increased rapidly in 4-day-old broiler,but they significantly decreased in 7-day-old broiler.Although the number of CD4+ cells had no marked change,their number kept increasing from 14-day-old to 35-day-old.The results revealed that the cellular immune function of caecal tonsil in chicken strengthened fast after hatching,and reached the mature level in 14 day-old broiler. |
|
|
|
Key words:chicken;caecal tonsil;T lymphocyte;subpopulation;development |
|
|
|
|
|
《中国兽医科学》 ©
版权所有
Chinese Veterinary Science
zgsykx@zgsykx.com |
|
