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硝基苯对小鼠脾的毒性作用
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刘伯臣1，邢厚娟2，丁岚峰1，徐世文2*
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（1.黑龙江民族职业学院，黑龙江 哈尔滨150081；
2.东北农业大学 动物医学院，黑龙江 哈尔滨150030）
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摘要：
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为探讨硝基苯对小鼠脾的毒性作用，采用灌胃的方法对试验小鼠用硝基苯染毒，染毒剂量分别为26mg/kg、52mg/kg、105mg/kg，每天染毒1次，共30d。于末次染毒后第2d将小鼠脱颈处死，立即取出脾检测抗氧化指标MDA、SOD、GSH-Px和T-AOC，并对其进行了显微和超微结构的观察。结果，随着染毒剂量的加大，MDA含量逐渐升高，SOD、GSH-Px酶活性和T-AOC逐渐降低；与油对照组相比，各剂量组均表现为差异极显著（P<0.01）；光镜下白髓数量减少，体积变小，生发中心不明显，高剂量组能见到少量的凋亡细胞；电镜下白髓区淋巴细胞染色质聚集，核周隙增宽，核边集现象增多，高剂量组可见个别细胞核膜破损，核内容物外溢。结果表明，硝基苯对脾具有明显的毒性作用，能够诱发机体氧化应激和脾淋巴细胞凋亡，氧化应激与细胞凋亡的发生存在一定关系。 |
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关键词：
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硝基苯；脾；显微结构；超微结构；抗氧化指标；小鼠 |
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中图分类号：S 859.82 文献标识码：
A 文章编号：1673-4696(2008)04-0355-05 |
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Toxic effect of nitrobenzene on spleen in mice |
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LIU Bai-chen1，XING Hou-juan2，DING
Lan-feng1，XU Shi-wen2 |
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(1.Heilongjiang College for Nationalities and Profession,Harbin 150081,China;
2.College of Veterinary Medicine,Northeast Agricultural University,Harbin 150030,China) |
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Abstract: In order to study the toxic effect of nitrobenzene(NB) on spleen in mice,26mg/kg,52mg/kg and 105mg/kg NB was daily administered intragastricly to the mice for 30d according to body weight of the mice.On day 2 post-final-administration,the mice were slaughtered and spleens of the mice were collected and dealt with a available method for the detection of antioxidant function and for the observation of microstructure and ultrastructure.MDA contents stepped up gradually ,SOD activity,GSH-Px activity and T-AOC stepped down gradually,and each NB group contrasted significantly with the oil-control（P<0.01）.Under the light microscope,white pulp diminished and shrank,germinal center was not clear and there were a little of apoptosis cell.Under the electron microscope,cyto-chromatin builtup in white pulp,perinuclear cisterna widened,nucleus gathered increasingly in cell edge,there were several damaged caryotheca,and nuclear content flowed over in the 105mg/kg NB group.The results indicated that NB could make the mice lymphocyte generate oxidative stress and induce cellular apoptosis,indicating that a certain relationship between oxidative stress and apoptosis might exist. |
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Key words: nitrobenzene(NB);spleen;microstructure;ultrastructure;antioxidant capacity;mice |
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