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吕氏泰勒虫和尤氏泰勒虫内转录间隔区基因序列的
测定与系统进化分析
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牛庆丽1，2,邱家祥2,关贵全1,马米玲1,刘志杰1,党志胜1，刘爱红1，高金亮1，
任巧云1，李有全1，刘军龙1，白启1，罗建勋1*，殷宏1*
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（1.中国农业科学院 兰州兽医研究所 家畜疫病病原生物学国家重点实验室 甘肃省动物寄生虫病重点
实验室，甘肃 兰州730046；2.新疆农业大学 动物医学学院，新疆 乌鲁木齐830052）
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摘要：
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自试验感染吕氏泰勒虫（渭源株）和尤氏泰勒虫（隆德株）绵羊的血液中纯化虫体，提取虫体基因组DNA，通过PCR扩增内转录间隔区(ITS1-5.8S-ITS2 rRNA)基因，然后进行测序，构建了系统发生树并与GenBank中各种动物梨形虫的ITS1-5.8S-ITS2 rRNA基因序列进行比较。结果显示，这两种泰勒虫的ITS1-5.8S-ITS2 rRNA基因大小为817～842bp，同源性高达96.8%，分布在同一大枝上。通过比较ITS基因的同源性，尤其是ITS2基因的变异性，可以区别这两种泰勒虫与其他梨形虫。 |
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关键词：
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系统进化分析;吕氏泰勒虫；尤氏泰勒虫；内转录间隔区基因 |
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中图分类号：S 852.723 文献标识码：
A 文章编号：1673-4696(2008)05-0365-05 |
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Sequencing and phylogenetic analysis of ITS genes of
Theileria luwenshuni and T.uilenbergi isolated in China |
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NIU Qing-li1,2,QIU Jia-xiang2,GUAN
Gui-quan1,MA Mi-ling1,LIU Zhi-jie1,DANG
Zhi-sheng1,
LIU Ai-hong1,GAO Jin-liang1,REN Qiao-yun1,LI
You-quan1,LIU Jun-long1,BAI Qi1,
LUO Jian-xun1,YIN Hong1 |
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(1.Key Laboratory of Animal Parasitology of Gansu Province/State Key Laboratory of Veterinary Etiological Biology/
Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046,China;
2.College of Veterinary Medicine,Xinjiang Agricultural University,rümqi 830052,China)
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Abstract: Theileria luwenshuni Weiyuan isolate and T.uilenbergi Longde isolate in China were purified from the blood of sheep inoculated experimentally with T.luwenshuni or T.uilenbergi,and their genomic DNAs were extracted.Then, the first internal transcribed spacer (ITS-1), 5.8S gene and the second internal transcribed spacer (ITS-2) of the two Theileria species were amplified by PCR,sequenced,and compared with those of other species of piroplasms available in GenBankTM.The results showed that the length of the ITS-1-5.8S gene and ITS-2 gene of the two Theileria species varied between 817bp and 842bp and their homology was 96.8%.The two Chinese isolates were in the same branch and could be diffe-rentiated with other piroplasms or between them by comparing the homology of ITS sequences,in particular the variation in ITS-2 sequences. |
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Key words: phylogenetic analysis;Theileria luwenshuni; T.uilenbergi; ITS1-5.8S-ITS2 gene |
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