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猪圆环病毒2型感染昆明小鼠模型的建立
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苗岚飞1,2 ，崔尚金1*，张超范1，胡守萍1，陈长木1，张垚1
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（1.中国农业科学院 哈尔滨兽医研究所 兽医生物技术国家重点实验室 猪传染病研究室，黑龙江
哈尔滨150001；2.东北农业大学 动物医学学院，黑龙江 哈尔滨 150030)
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摘要：
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将SPF级昆明小鼠随机分为3组，即一次攻毒组（S组）12只、连续攻毒组（M组）3只和对照组（C组）12只。S组和C组小鼠于第1d分别接种RPMI 1640培养液和猪圆环病毒2型（PCV2）细胞培养毒，M组小鼠于第1、7、14、28、35d连续进行PCV2接种。S组和C组小鼠分别在接种后第7、14、28和42d各处死3只，M组小鼠在接种后第42d全部处死，分析其增重、病毒组织分布、抗体水平、病理组织学变化。结果，C组小鼠体重增长速率大于S组和M组，S组又明显高于M组；S组和M组小鼠体内存在病毒复制，而C组小鼠体内无病毒复制；S组和M组小鼠均产生PCV2抗体，但M组小鼠抗体水平明显高于S组，C组小鼠未检测到PCV2抗体；S组和M组小鼠有明显的病理损伤，其中淋巴结损伤最严重，C组未发现病理损伤。结果表明PCV2可以成功感染SPF昆明小鼠。 |
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关键词：
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SPF昆明小鼠；猪圆环病毒2型；病理损伤；动物模型 |
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中图分类号：S 852.659.2 文献标识码：
A 文章编号：1673-4696(2008)06-0475-04 |
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Establishment of a Kunming mouse model for infection with PCV2 |
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MIAO Lan-fei1,2,CUI Shang-jin1,ZHANG
Chao-fan1,HU Shou-ping1,CHEN Chang-mu1,ZHANG
Yao1
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(1.National Key Laboratory of Veterinary Biotechnology/Division of Swine Infectious Diseases,Harbin
Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150001,China;
2.College of Veterinary Medicine,Northeast Agricultural University,Harbin 150030,China) |
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Abstract: Twelve SPF-Kunming mice in Group S were inoculated with RPMI 1640,12 SPF-Kunming mice in the control were inoculated with a single dose of porcine circovirus type 2(PCV2) and 3 SPF-Kunming mice in Group M were injected with multiple doses of PCV2.Three mice in the control and 3 mice in Group S were sacrificed on day 7,14,28,and 42 post-inoculation(PI),respectively.All mice in Group M were sacrificed on day 42 PI.Their body weights and levels of antibody against PCV2 were examined.The increment of body weight in the control was significantly higher than those in Group S and M,and the increment of body weight in Group S was significantly higher than that in Group M.The viral replication was detected in Group S and M,but not detected in the control.The specific antibody against PCV2 were observed in Group S and M.The level of antibody in Group M was significantly higher than that in Group S.No anti-PCV2 antibody was detected in the control.The conspicuous pathological injuries were observed in Group S and M.The lymph nodes of the mice in Group S and M were damaged seriously by PCV2.The same injury was not found in the control.The results showed that mice could be infected successfully by PCV2. |
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Key words: SPF-Kunming mouse;porcine circovirus type 2;pathological injury;animal model |
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