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用日本血吸虫重组抗原诊断牛血吸虫病的研究
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周伟芳,林矫矫*,朱传刚,石耀军，刘金明
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(中国农业科学院 上海兽医研究所 国家防治血吸虫病专业实验室 农业部动物寄生虫学
重点开放实验室,上海200232)
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摘要：
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为寻找可替代虫卵抗原(SEA)用于家畜血吸虫病血清学诊断的重组抗原，采用酶联免疫吸附试验（ELISA）比较了日本血吸虫23ku膜蛋白大亲水区多肽重组蛋白LHD-Sj23和日本血吸虫SEA检测牛血吸虫病的敏感性和特异性。结果显示，SEA和LHD-Sj23作为诊断抗原对189例血吸虫病牛和92例健康牛的阳性检出率分别为87.8%和90.5%，阳性符合率分别为93.5%和92.4%。两种抗原之间敏感性和特异性均无显著性差异(P>0.05)。提示，LHD-Sj23重组抗原可替代SEA用于家畜血吸虫病的血清学诊断。 |
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关键词：
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牛血吸虫病；重组抗原；血清学诊断 |
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中图分类号：S 852.723
文献标识码：
A 文章编号：1673-4696(2008)06-0489-05 |
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Studies on recombinant 23ku membrane protein from Schistosoma japonicum
as antigens in ELISA for serodiagnosis of bovine schistosomiasis |
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ZHOU Wei-fang,LIN Jiao-jiao,ZHU Chuan-gang,SHI Yao-jun,LIU Jin-ming |
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(National Key Laboratory of Animal Schistosomiasis Control/Key Laboratory of Animal Parasitology of the Ministry of
Agriculture/Shanghai Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Shanghai 200232,China) |
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Abstract: To evaluate the potential of recombinant 23ku membrane protein of Schistosoma japonicum(Sj23)-produced in the Escherichia coli as a substitute for soluble egg antigen(SEA) as coating antigen in serodiagnosis of bovine schistosomiasis,the sensitivity and specificity of recombinant Sj23 and SEA in ELISA were compared using 189 bovine sera with schistosomiasis and 92 bovine sera without schistosomiasis.The result showed that the sensitivity and specificity of ELISA using SEA as coating antigen were 878% and 93.5%,respectively,and the sensitivity and specificity of ELISA using recombinant Sj23 as coating antigen were 90.5% and 92.4%,respectively.No significant difference was found between the two ELISAs on sensitivity and specificity,indicating that the recombinant Sj23 could be used as antigen to replace SEA in serodiagnosis of bovine schistosomiasis. |
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Key words: bovine schistosomiasis;recombinant antigen;serodiagnosis |
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